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The prince shows incipient signs of an H+ facies - Anne does not, she did not inherit the H+ mutation
"Anne or Ana de Austria (1601-1666) at age 9 yrs (future Queen of France) and Phillip IV at age 5 yrs (Future Emperor of Spain)" (see details) (see related A) Bartolome Gonzalez y Serrano (1564-1627)
See other companion images for comapanion notes. PLEASE NOTE companion videos - "Game of thrones - Bastards - Emperors - Genes". Please also see companion images which contain many other comments. One central question is "who is the real father of the Emperor Carlos I or Charles V.